摘要
目的探讨结肠清洁联合善宁治疗犬急性坏死性胰腺炎(ANP)时促炎因子的变化。方法健康杂种犬16只,随机分为4组:(1)假手术组(A组,n=4);(2)ANP组(B组,n=4);(3)善宁治疗组(C组,n=4);(4)善宁+结肠清洁联合治疗组(D组,n=4)。采用胰管内注入5%牛磺胆酸钠(1ml/kg体重)和胰蛋白酶(每毫升含8000~10000BAEE)混合液的方法诱导犬ANP,以0.9%氯化钠注射液取代5%牛磺胆酸钠混合液即为假手术组。每只犬均在术前30min、术后8h、26h、50h、74h各取股静脉血2ml,测定TNF-α、IL-β、IL-6、IL-8的动态变化,术后12h、24h、48h、72h测定血清淀粉酶,术后74h处死犬。结果血清淀粉酶的变化符合ANP。组间比较显示,术后促炎因子水平联合治疗组明显低于善宁治疗组和ANP组(P〈0.05),善宁治疗组明显低于ANP组,ANP组和善宁治疗组均明显高于假手术组(P〈0.05)。结论结肠清洁联合善宁治疗ANP对促炎因子清除明显。
Objective To explore a combined treatment of colonic therapy with Sandostatin for severe acute pancreatitis and observe dynamic changes of pro-inflammatory cytokines in an animal model. Methods Sixteen healthy mongrel dogs were randomly divided into normal control group(n -= 4), severe acute pancreatitis group(n=4), Sandostatin therapy group(n = 4), and Sandostatin plus colonic therapy group(combined therapy group, n = 4). Severe acute pancreatitis was induced in dogs by pancreatic duct infusion of a mixture of 5% sodium taurocholate (1 ml/kg) and trypsin(8 000 10 000 BAEE in every milliliter). The mixture of sodium taurocholate was replaced by by 0.9% sodium chloride in normal control group. 2 ml blood was drawn from the femoral vein 30 min before operation, and 8, 26, 50 and 74 h after operation. Dynamic changes of tumor necrosis factor-α(TNF-α), interleukin 1β(IL-1β), interleukin-6(IL-6) and interleukin-8(IL-8) were tested. Serum amylase levels were observed at 12, 24, 48 and 72 h. The dogs were killed at 74 h after operation. Results Changes of serum amylase level were consistent with SAP. Inter-group comparison showed that plasma proinflammatory cytokine level was significantly lower in combined therapy group than in Sandostatin therapy group and SAP group after operation, and that plasma pro-inflammatory cytokine level was significantly lower in Sandostatin therapy group than in SAP group, and significantly higher in Sandostatin therapy group and SAP group than in normal control group (P 〈 0. 05). Conclusions Colonic therapy combined with Sandostatin has a significant effect of clearing pro-inflammatory cytokines.
出处
《胰腺病学》
2006年第1期30-32,共3页
Chinese JOurnal of Pancreatology