免疫相关转录因子GATA-3基因在再障和正常骨髓基质细胞中的表达

Expression of transcription factor GATA-3 gene in bone marrow stromal cells from patients with aplastic anemia and normal controls

目的:了解转录因子GATA-3基因在再生障碍性贫血(AA)和正常骨髓基质细胞(BMSC)中表达的情况,探讨AA发病的免疫机制。方法:采集9例AA患者的骨髓标本及34例正常骨髓标本,分离骨髓单个核细胞后体外扩增培养骨髓基质细胞,运用RT-PCR-ELISA方法检测GATA-3基因的表达程度,并对其相对表达水平进行半定量分析。结果:GATA-3基因在正常和再障的骨髓基质细胞中均有一定的表达。再障的骨髓基质细胞中GATA-3的表达水平较正常高。结论:转录因子GATA-3基因可在正常和再障骨髓基质细胞中表达,并可能影响机体免疫功能及骨髓微环境的造血调控,参与AA发病的免疫机制。

AIM： To investigate the expression of transcription factor GATA - 3 gene in the bone marrow stromal cells （BMSCs） from patients with aplastic anemia （AA） and normal controls. METHODS： The expression of GATA- 3 gene was analyzed by using RT - PCR - ELISA in BMSCs from 34 normal cases and 9 cases with AA. The standardized semi - quantitative expression level of GATA- 3 gene in BMSCs from patients with AA was compared with normal controls. RESULTS： The expression of GATA - 3 gene was detected in BMSCs from both normal controls and the cases with AA. The expression level of GATA - 3 gene in BMSCs from AA was significant higher than that from the normal controls （ P 〈 0.05）. CONCLUSION： The expression of GA- TA - 3 gene was identified in BMSCs from normal controls and patients with AA. It may influence the function of immunity and the regulation of hematopoiesis in BMSCs. It may play the role in pathogenesis and development of AA.