缺血预处理对大鼠缺血性脑损伤线粒体钙、细胞色素C水平的影响

Effect of ischemic preconditioning on contents of cytochrome C and mitochondrial calcium in rats after focal cerebral ischemic-reperfusion

目的:观察缺血预处理对大鼠缺血性脑损伤后线粒体钙、细胞色素C水平的影响。方法:用大鼠右侧大脑中动脉阻闭制成局灶性脑缺血模型。24只大鼠,每组8只,随机分为缺血预处理组、模型组和假手术组。缺血预处理组于3d前给予30min预缺血及72h再灌注,实验时行2h缺血4h再灌注。用张均田的改良方法测定细胞色素C含量。用火焰原子吸收法检测线粒体钙含量。结果:缺血预处理组动物的细胞色素C、线粒体钙与假手术组相比差异显著(P<0·05,P<0·01),缺血预处理组与模型组相比差异显著(P<0·05,P<0·01)。结论:缺血预处理减少线粒体释放细胞色素C,维持线粒体钙稳态。

AIM： To observe the effect of ischemic preconditioning on contents of cytochrome C and mitochondrial calcium in rats ＂after focal cerebral ischemic - reperfusion. METHODS： Focal cerebral ischemic model was made by occlusion of right middle artery in Wistar rats （ischemia for 2 h and reperfusion for 4 h）. Rats were randomly divided into three groups： ischemia pretreatment, model and sham operation. Rats in isehemic pretreatment group were undergone transient ischemic preconditioning （30 min） and reperfusion （72 h）. The contents of cytochrome C were measured according to Zhangjtmtian＇ s improved methods. The contents of mitochondrial calcium were detected by flame atom absorption. RESULTS： The contents of mitochondrial cytochrome C and calcium in model group were significantly lower than those in sham operation （P 〈 0.05, P 〈 0.01 ）, whereas the contents of plasma cytochrome C were markedly higher （P 〈 0.05, P 〈 0.01 ）. The change in ischemic pretreatment group was obviously difference as compared with the model group. CONCLUSION： Ischemic preconditioning reduces the release of mitochondrial cytochrome C and maintain mitochondrial calcium homeostasis.