血小板反应蛋白-1及受体CD36在肝细胞性肝癌中的表达及意义

Significance of expression of thrombospondin-1 and receptor-CD36 in hepatocellular carcinoma

目的:研究肝细胞性肝癌(HCC)中血小板反应蛋白-1及受体CD36的表达及其与侵袭转移和血管生成的关系。方法:应用逆转录聚合酶链反应(RT-PCR)和免疫组织化学(SP法)法检测43例肝癌和癌旁组织中血小板反应蛋白-1、CD36的表达、CD34标记微血管密度,并分析其与肝癌临床病理特征及相互之间的关系。结果:肝癌中血小板反应蛋白-1的阳性率低于癌旁组织,与有癌栓、包膜不完整、侵袭转移能力强呈负相关;CD36的表达与有癌栓、侵袭转移能力强呈负相关,两者呈正相关,并且两者阳性组的微血管密度均显著低于阴性组。结论:血小板反应蛋白-1可抑制肝癌的生长、延缓侵袭转移、抑制血管生成,受体CD36可能是血小板反应蛋白-1的作用途径之一。

AIM： To study the expression of thrornbespondin - 1 （TSP - 1 ） and receptor- CD36, and investigate the relationship between tumor invasive capability and microvessel density and thrornlzospondin- 1. METHODS： 43 hepatocellular carcinoma （HCC） cases were under investigation. Tissues from tumor, corresponding adjacent non- HCC tissue were stained with CD34 to show the MVD. TSP- 1 and CD36 were examined by immunohistochemistry （SP） and RT- PCR. Relationship between clinical pathological features and above parameters was analyed. RESULTS： The staining of TSP- 1 in HCC tissue is significantly lower than that in corresponding adjacent non- HCC tissue. Expression of TSP- 1 was correlated to tumor thrombi, capsule, tumor invasive capability and CD36. CD36 was also correlated to tumor thrombi and tumor invasive capability. MVD was significantly higher in TSP- 1, CD36 positive group than that in negative group. CONCLUSION： TSP- 1 inhibits the growth, invasion and angiogenesis in HCC. TSP- 1 may take effect through CD36.