塞来昔布对大鼠心脏移植急性排斥反应的抑制作用

Effects of celecoxib on the apoptosis in acute cardiac allograft rejection in rats

目的观察塞来昔布对大鼠心脏移植急性排斥反应的抑制作用。方法以SD大鼠为供者,Wistar大鼠为受者,进行40次腹部异位心脏移植。采用HE染色和原位末端标记(TUN EL)技术检测移植心切片,进行排斥反应的病理分级并计算移植心肌细胞的凋亡指数(AI)。结果移植心的细胞凋亡主要发生于心肌细胞;移植后第3、5d,塞来昔布治疗组心肌细胞凋亡指数分别为:1.03±0.42和3.28±2.42;对照组分别为2.35±1.51和11.35±3.46;两组比较,差异有统计学意义(P<0.001)。结论细胞凋亡是心脏移植急性排斥中组织损伤的重要机制;塞来昔布能明显抑制心肌细胞凋亡。

Objective To investigate the effects of celecoxib on apoptosis during acute rejection of cardiac allograft in rats. Methods Forty Wistar rats underwent heterotopic heart transplantation in the abdominal portion from disparate SD rats. The cardiac grafts were harvested at 3rd and 5th day after transplantation. The cardiac allograft sections were subjected to the HE staining and in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling （TUN EL） for histopathological rejection grade and apoptotic index （AI）. Results Apoptosis mainly occurred in cardiac myocytes throughout allograft rejection. Celecoxib could markedly reduce myocyte apoptosis and abate the damage to the graft tissue. The AI at 3rn and 5th day post-transplantation were 2. 35 ± 1.51 and 11.35 ± 3.46 in control group, and 1.03 ± 0. 42 and 3.28 ± 2. 42 in celecoxib group respectively with the difference between them being significant. Conclusions Apoptosis is an important mechanism of cell death in acute cardiac allograft reiection in rats. Celecoxib can apparently inhibit the apoptosis of cardiac myocytes, which may be an important way of its immunosuppressive actions.