大鼠应激性溃疡自愈过程中环氧合酶表达的变化

Changes in cyclooxygenase gene expression during spontaneaus recovery from stress ulcer in rats

目的研究大鼠应激性溃疡自愈过程中环氧合酶的表达;探讨环氧合酶在应激性溃疡自愈过程的作用机制。方法应用免疫组织化学和RT．PCR方法检测大鼠应激性溃疡自愈过程中环氧合酶蛋白和mRNA的表达变化。结果对照组的大鼠胃粘膜COX-2表达极低,而在溃疡自愈过程中其表达显著增加(P<0．05),并随自愈时间而减弱;COX-1在应激性溃疡自愈各组和对照组中均表达,自愈各组与对照组相比没有明显差异(P>0．05)。结论大鼠应激性溃疡自愈过程中COX-1和COX-2均有表达。参与了应激性溃疡的自愈过程,其作用可能主要与COX介导分泌的前列腺素有关。

Objective To observe the changes in gene expression of cyclooxygenase （COX） during spontaneaus recovery from stress ulcer in rats exposed to water immersion and restraint stress （WRS）. Methods A rat model of stress ulcer was established by means of WRS, in which the changes in COX expression were detected with immunohistochemistry and reverse transcription （RT）-PCR. Results Very low levels of COX-2 expression were detected in the gastric mucosa of the control rats, and the expression increased significantly during the healing process of the stress ulcer （P〈0.05）. COX-1 expression in the gastric mucosa showed no significant difference between the control group and the stress ulcer groups during healing （P〉0.05）. Conclusion COX-1 and COX-2 expressions in rat gastric mucosa during the recovery from stress ulcer participate in the recovery of the damaged mucosa possibly by mediating prostaglandin secretion.