CD25^+细胞在鼻咽癌中的表达及与EBV的关系

Expression of CD25^+ lymphocytes in nasopharyngeal carcinoma and its association with EBV infection

目的探讨CD25+细胞在鼻咽癌组织中的表达情况及EB病毒感染对CD25表达的影响。方法用免疫组织化学方法检测CD25+细胞在鼻咽癌组织和鼻咽慢性炎症组织中的表达情况,并用组织原位杂交技术检测EB病毒对其表达的影响。对照组选取鼻咽慢性炎症组织。结果CD25+淋巴细胞在鼻咽癌组织中比在鼻咽慢性炎症组织中的表达明显升高, 差异具有显著性意义(P<0．001),且在未分化癌中表达高于角化型癌和非角化型癌(P<0．05)。原位杂交显示EB病毒与 CD25+淋巴细胞高表达有关。结论 CD25+淋巴细胞在鼻咽癌组织中表达高于慢性炎症组织,EB病毒感染与CD25+淋巴细胞表达间存在相关性。

Objective To investigate the expression of CD25^＋ lymphocytes in nasopharyngeal carcinoma （NPC） tissue and the influence of Epstein-Barr virus （EBV） infection on CD25 expression. Methods Immunohistochcmistry was used to detect CD25 expression in the NPC tissues and in situ hybridization employed to detect EBV infection with chronic nasopharyngitis tissue as the control sample. Results Significant difference was noted in the expression of CD25^＋ lymphocytes between NPC and chronic inflammatory tissues. The expression was higher in undifferentiated NPC than in keratinizing squamous cell carcinoma and non-keratinizing carcinomas. The NPC tissue was all EBV-positive except for one sample,, which was identified as keratinizing carcinoma, but the control samples were all negative for EBV infection, which was correlated with CD25 expression. Conclusion The expression of CD25^＋ lymphocytes is higher in NPC tissues and correlated to EBV infection.