SYBR荧光实时定量PCR检测肝移植受者CD95和CD95L mRNA表达

Detection of CD95 and CD95L mRNA expression after liver transplantation using SYBR realtime PCR during acute rejection

目的检测肝移植受者外周血淋巴细胞CD95和CD95L mRNA的表达,并探讨其在移植肝急性排斥反应中的意义。方法采用SYBR荧光实时定量PCR法检测56例肝移植受者外周血淋巴细胞CD95和CD95L mRNA的表达。结果 CD95和CD95L mRNA在移植肝急性排斥患者外周血中的表达均显著升高(P<0．01),时间上平均早于血肝功能酶谷丙转氨酶、谷草转氨酶升高2 d。结论 CD95和CD95L与移植肝急性排斥反应密切相关,CD95和CD95L mRNA可作为移植肝急性排斥反应发生的预测和诊断指标之一。

Objective To detect the expression of CD95 and CD95L mRNAs after liver transplantation and investigate the role of CD95 and CD95L in acute liver allograft rejection. Methods The expressions of CD95 and CD95L mRNAs of peripheral blood lymphocyte from 56 liver allograft recipients were examined using SYBR real-time PCR. Results CD95 and CD95L mRNA levels in the recipients with acute rejection were significantly higher than those without rejection （P〈0.01）, and the elevation occurred about 2 days earlier than that ofalanine aminotransferase and aspartate aminotransferase. Conclusion CD95 and CD95L are related to acute liver allograft rejection and their mRNA expression Ttevel may serve as an indicator for predic- tion and diagnosis of acute rejection episodes.