家族性食管癌组织中FHIT、p53蛋白的表达

Expression of FHIT and p53 in patients with familial esophageal cancer

目的:探讨家族性食管癌组织中脆性组氨酸三联体基因(FHIT)和p53蛋白的表达。方法:采用免疫组织化学ABC法对74例河南省林州市食管癌患者(其中33例家族性食管癌,41例非家族性食管癌)手术切除标本癌及癌旁正常组织中FHIT及p53蛋白进行检测。结果:食管癌组织中FHIT蛋白异常表达率(39%,29/74)高于癌旁正常组织(3%,1/31),家族性食管癌组织中FHIT蛋白异常表达率(56%,18/33)高于非家族性食管癌组织(27%,11/41)(P均<0.05)。家族与非家族性食管癌组织p53蛋白阳性表达率差异无统计学意义(P>0.05)。结论:FHIT异常表达可能与食管癌家族高易感性密切相关;p53可能与其他易感因素协同作用,影响食管癌的发生。

Aim ： To explore the molecular changes of FHIT and p53 protein in familial esophageal carcinoma （ EC）. Methods： Seventy four EC patients, including 33 familial EC and 41 non-familial EC, were enrolled to determine FHIT, p53 protein using immunohistochemistry. Results： The abnormal expression of FHIT gene in familial EC （56% , 18/33 ） was significantly higher than that in non-familial EC （27% , 11/41 ） （P 〈 0.05 ）. Aberrant expression of FHIT protein in EC tissue （39% ,29/74） was much higher than that in normal tissue （3%, 1/31 ） （P 〈 0.05 ）. Although the aberrant expression rate for p53 in familial EC （52% , 17/33 ） was higher than in non-familial EC （46% , 19/41 ） , the difference was not significant （ P 〉 0.05 ）. Conclusion ： FHIT may play an important role in the EC familial hereditary susceptibility, and p53 may be an important synergistic agent in the process.