食管癌和淋巴结转移组织中c-myc,hTERT和c-MET蛋白的表达

Expression of c-myc, hTERT and c-MET in esophageal squamous cell carcinoma and lymph node metastatic tissue

目的:探讨食管癌和淋巴结转移组织中c-myc、hTERT和c-MET的表达。方法:利用组织微阵列技术,采用免疫组化ABC法,测定58例食管癌及其相应的癌旁组织、8例转移淋巴结组织中c-myc、hTERT和c-MET的表达。结果:癌旁、癌及淋巴结转移组织中c-myc阳性率分别为7%,66%,75%;hTERT阳性率分别为0%,48%,75%;c-MET阳性率分别为7%,34%,75%;癌旁和癌组织间c-myc和hTERT的表达差异有统计学意义(P<0.05),癌旁和癌组织间,以及癌和淋巴结转移灶之间c-MET的表达差异均有统计学意义(P<0.05)。食管癌组织中c-myc与hTERT的表达呈正相关(r=0.411,P=0.002),c-myc与c-MET的表达不相关(r=0.211,P=0.146)。结论:c-myc、hTERT和c-MET的高表达可能与食管癌发生发展相关,c-myc可能参与了hTERT的激活过程,c-MET的高表达可能参与了食管癌淋巴结的转移过程。

Aim： To explore the role of c-myc, hTERT, and c-MET in esophageal squamous cell carcinoma （SCC） development and progression. Methods： A total of 58 cases of SCC, the corresponding adjacent normal epithelium, and 8 metastatic lymph node lesions were enrolled to detect the protein expression of c-mye, hTERT, and c-MET using tissue array technique and immunohistochemical ABC method. Results： The positive expression rate of c-myc in SCC, adjacent lesions, and metastatic lymph node lesions were 66% , 7% , and 75% , respectively. The positive expression rates of hTERT were 0% , 48%, and 75% in adjacent lesion, cancer samples and metastatic lymph node lesions, respectively, There were evident differences in the expression rates of c-myc or hTERT between the adjacent lesion and cancer lesions （P 〈 0.05）. The positive rate of c-MET were 7% , 34% and 75% in adjacent lesion, cancer samples and metastatie lymph node lesions, respectively ,and there was evident difference between adjacent lesions and cancer samples and between cancer samples and metastatic lymph node lesions （P 〈0.05）. The expression of c-mye was positively correlated with that of hTERT（r =0.411 ,P = 0. 002） , and was not correlated with that of c-MET（r = 0.211 ,P = 0. 146） in SCC tissue. Conclusion ： c-mye, hTERT and c-MET may be involved in SCC development and progression, c-myc may be involved in activating process for hTERT, and c- MET may be involved in lymph node metastasis in SCC.