食管癌前病变和癌组织中p15、p16、mdm2和p53蛋白的表达

Expression of p15,p16,mdm2,and p53 protein in different precancerous lesion and esophageal carcinoma tissue

目的:探讨食管癌高发区河南林州市食管癌患者p15、p16、mdm2和p53的表达变化特征及其与各级病变的关系。方法:取食管癌高发区河南林州市居民156例的食管内镜黏膜活检组织(包括正常食管上皮(NOR)21例,基底细胞过度增生(BCH)45例,不典型增生(DYS)23例,原位癌组织(CIS)45例和鳞状细胞癌(SCC)22例),采用免疫组化ABC法分析p15、p16、mdm2和p53蛋白的表达情况。结果:NOR和各级癌前病变组织及SCC组织中均出现不同程度的p15、p16、mdm2和p53表达。随着病变发展(从NOR→BCH→DYS→CIS→SCC),p15和p16的表达逐渐降低,mdm2和p53的表达逐渐增高(P<0.05)。结论:p15、p16的低表达和p53、mdm2的高表达可能是导致病变持续向癌方向发生发展的机制之一。

Aim： To characterize the changes of P15, p16, mdm2 and p53 expression in esophageal normal epithelial tissue and different precancerous lesions and esophageal carcinoma. Mothods： A total of 156 esophageal biopsy samples, including 21 cases of normal esophageal epithelia （NOR） ,45 cases of basal cell hyperplasia （BCH） , 22 cases of dysplasia （DYS） , 45 cases of carcinoma in situ （CIS） , and 22 cases of esophageal squamous cell carcinoma （SCC） , were subjected to determine the expression of p15, p16, mdm2 and p53 using immunohistochemical ABC method. Results： With the lesions progressed from NOR→BCH→DYS→CIS→SCC, the positive rates of p15 and p16 decreased （ P 〈 0.05） , and the positive rates of mdm2 and p53 increased significantly（ P 〈 0.05 ）. Conclusion： The low-expression of P15, p16 and high- expression of mdm2 and p53 may be one of the mechanisms to drive the mild lesions towards carcinogenesis.