骨髓间质干细胞分离培养及向心肌细胞的转化

Differentiation of bone marrow mesenchymal stem cells into cardiomyocyte

目的:探讨骨髓间质干细胞(MSCs)诱导分化为心肌细胞的可能性,为干细胞移植治疗心肌梗死寻求理想的细胞材料。方法:体外分离培养大鼠骨髓MSCs,5-氮胞苷(5-aza)诱导24h后继续培养,免疫细胞化学染色和RT-PCR检测心肌细胞特异性蛋白的表达。结果:MSCs经5-aza诱导后3周心肌肌钙蛋白T和连接蛋白43免疫细胞化学染色阳性表达;RT-PCR显示诱导3周细胞有心肌肌钙蛋白I、α心肌肌动蛋白表达。结论:MSCs可在体外经5-aza诱导定向分化为心肌细胞。

Aim ： To study the possibility of bone marrow stem cells（MSCs） differentiating into cardiomyocytes and provide ideal transplanting stem cells in myocardial infarction therapy. Methods： Rat MSCs were separated and cultured. Culture of MSCs was maintained after being induced by 5-aza for 24 h. Cardiomyocytes were identified by immunocytochemistry staining and RT-PCR. Results： Immunocytochemistry results showed that cardiac Troponin T（cTnT） and connexin- 43 expressed in MSCs at 3 weeks after induction by 5-aza. RT-PCR result suggested that cardiac Troponin I（cTnI） and α- cardiac actin expressed in MSCs at 3 weeks after induction by 5-aza. Conclusion： MSCs could differentiate into cardiomyocytes after being induced by 5-aza in vitro.