丹皮酚增强顺铂对人肝癌细胞SMMC-7721的增殖抑制作用

Synergistically suppressive effect of paeonol and cisplatin on the proliferation of human hepatoma cell line SMMC-7721

目的探讨丹皮酚和顺铂联合应用对人肝癌细胞SMMC07721的增殖抑制作用。方法用不同浓度的丹皮酚、顺铂和两药联用处理人肝癌细胞SMMC-7721，采用四甲基偶氮唑盐（MTT）法测定药物在体外对人肝癌SMMC07721细胞的增殖抑制作用，应用两药相互作用指数（CDI）进行联合用药分析。结果丹皮酚（7．81-250mg／L）和顺铂（0．625～20mg／L）单独应用均对人肝癌SMMC-7721细胞有抑制作用，呈现剂量效应关系。丹皮酚与顺铂联合应用后对人肝癌SMMC-7721细胞的抑制率明显大于单药，有显著的协同杀伤作用。结论丹皮酚与顺铂联合应用具有明显的协同抗肝癌细胞株SMMC-7721增殖的作用。

Objective To investigate the synergistically suppressive effect of paeonol and cisplatin on the proliferation of human hepatoma cell line SMMC-7721. Methods SMMC-7721 cells were cultured in regular condition and incubated with different concentrations of drugs. Cytotoxicity of the drugs was measured by MTT assay. The coefficient of drug interaction（CDI） was used to analyse the synergistically suppressive effect of drug combination. Results Paeonol（7.81 -250 mg/L） and cisplatin （0. 625 -20 mg/L） had inhibitory effects on the proliferation of human hepatoma cell line SMMC-7721 in dose-dependent manner. The inhibitory rates of SMMC-7721 treated with combined agents were significantly higher than-those treated with Paeonol and cisplatin alone. The synergistically suppressive effect was significant. Conclusion The results suggest that the combination of paeonol and cisplatin has obviously synergistically inhibitory effect on the proliferation of human hepatoma cell line SMMC-7721.