急性脑梗死患者TLR4 mRNA表达与TNF-α相关性研究

Correlation between TNF-α concentration and TLR4 mRNA expression in patients with acute cerebral infarction

目的观察急性脑梗死患者Toll样受体4(TLR4)mRNA表达及与TNF-α浓度的相关性,初步探讨TLR4在急性脑梗死缺血炎性损伤中的作用,为临床治疗脑梗死提供新的思路。方法分别运用RT-PCR法、ELISA法测定35例急性脑梗死患者的外周血单个核细胞(淋巴细胞、单核细胞)TLR4mRNA表达及血清TNF-α浓度,同期与20例正常者、20例动脉粥样硬化患者比较。结果脑梗死组发病第三天TLR4mRNA表达、血清TNF-α浓度均显著高于正常对照组和动脉粥样硬化组(P<0.01)。TLR4mRNA表达与血清炎性因子TNF-α浓度呈正相关(P<0.01)。结论脑梗死患者急性期外周血单个核细胞TLR4mRNA表达上调,可能通过促使炎性因子产生分泌增多来介导脑缺血炎性损伤。

Objective To study the correlation between the mRNA expression of Toll-like receptor 4 （TLR4） and the concentration of TNF-α in acute cerebral infarction, in order to explore primarily the role of TLR4 in the inflammatory injury of acute cerebral infarction, and provide a new way for the management of acute cerebral infarction. Methods RT-PCR and ELISA were respectively used to measure the mRNA expression of TLR4 in the peripheral lymphocytes and monocytes, the concentration of TNF-α in the serum. Results The mRNA expression of TLR4 and the concentration of TNF-α in infarction group were significantly higher than those of atherosclerotic group and healthy group at the 3rd day after acute cerebral infarction onset （P〈0.01）, and the expression of TLR4 mRNA was correlated positively with the concentration of TNF-α （P〈0.01）. Conclusion The expression of TLR4 mRNA in the peripheral lymphocytes and monocytes during acute cerebral infarction are elevated, which may play a critical role in the mechanism of cerebral inflammatory injury through spuring the excretion of inflammatory factor TNF-α.