摘要
目的:研究小分子干扰RNA片段(siRNA)对舌癌多药耐药(MDR)细胞系KBv200细胞的MDR1基因表达及其功能的影响,探讨siRNA作为未来化疗增敏剂的可能性。方法:运用针对MDR1基因序列的siRNA(MDR1-siRNA)在脂质体的介导下转染KBv200细胞;用RT-PCR分析MDR1 mRNA的表达水平;流式细胞术检测P-糖蛋白(P-gp)的表达;荧光分光光度法检测细胞内多柔比星(Dox)的积蓄;MTT法检测KBv200细胞对长春新碱(VCR)和Dox的敏感性变化。结果:MDR1-siRNA作用24和48 h能抑制MDR1基因的表达(P<0.05),其mRNA水平和P-gp水平均明显下降,同时使KBv200细胞对VCR和Dox的敏感性显著增加(P<0.01),并显著增加细胞内Dox的蓄积(P<0.01)。结论:MDR1-siRNA能显著抑制KBv200细胞内MDR1基因介导的MDR。
Objective ： To study the effect of siRNA on muhidrug resistancel （ MDR1 ） gene expression in KBv200 cells. Methods： MDR1-siRNA was synthesized and transfected into KBv200 cells. The MDR1 mRNA and P-gp were assayed by RT-PCR and flow cytometry. Cytotoxicity was determined by a tetrazolium-based chemosensitivity assay （MTF）. Cellular accumulation of Dox was measured by fluorescence spectrophotometry. Results： MDRI-siRNA decreased MDR1 mRNA and P-gp expression（P 〈 0. 05） , increased the sensitivity of KBv200 cells to VCR and Dox（P 〈0.01 ） , and also increased cellular Dox accumulation（P 〈 0.01 ）. Conclusion： MDRI-siRNA can reverse the muhidrug resistance to VCR and Dox in KBv200 cells.
出处
《医学研究生学报》
CAS
2006年第2期104-106,110,共4页
Journal of Medical Postgraduates
基金
国家留学基金资助项目(批准号:22832107)
