丙型肝炎病毒持续感染对体外培养人胎盘合体滋养层细胞生物学性状的影响

Effects of hepatitis C virus persistent infection on biological behavior of human placental syncytiotrophoblast in vitro

目的:探讨丙型肝炎病毒(HCV)持续感染对体外培养人胎盘合体滋养层细胞生物学性状的影响。方法:分别采用M atrigel人工重建基膜侵袭实验、MTT法细胞黏附实验、细胞移动实验,研究HCV RNA阳性患者血清感染体外培养的人胎盘滋养层细胞侵袭力以及与侵袭力相关的黏附、移动能力的改变;检测培养上清中人绒毛膜促性腺激素(HCG)浓度以评估感染对细胞激素合成、分泌功能的影响。结果:感染组细胞侵袭、黏附、移动以及激素合成分泌功能较对照组均有显著下降(P<0.05)。结论:HCV持续感染可抑制体外培养人胎盘合体滋养层细胞包括侵袭力和激素合成、分泌功能在内的多种生物学功能。

Objective ： To investigate the effect of HCV persistent infection on biological behavior of human placental syncytiotrophoblast in vitro. Methods： Using Matrigel invasion assay, cell adhesive assay and migration assay to measure the effect of HCV persistent infection on invasive and relative adhesive and migration ability of human placental syncytiotrophoblast in vitro. Content of HCG in supernatant of the cultured medium was detected to evaluate the change of hormone synthesis and secretion of the infected syncytiotrophoblast. Results ： Ability of invasion, adhesion, migration and function of hormone synthesis and secretion of infected syncytiotrophoblast decreased significantly in comparison with cells of control group （ P 〈 O. 05 ）. Conclusion ： HCV persistent infection suppressed the biological behavior of human placental syncytiotrophoblast in vitro.