慢性肾功能不全患者血清抗β1肾上腺素受体和M2胆碱能受体自身抗体的研究

Study on the roles of autoantibodies against β1 adrenoceptor and M2 cholinergic receptor in patients with chronic renal insufficiency

目的探讨抗β1-受体和M2-受体自身抗体与慢性肾功能不全的关系。方法以合成的受体多肽片段为抗原,应用ELISA技术检测了76例慢性肾功能不全患者,高血压60例,对照组40例血清中抗β1和M2受体自身抗体。结果慢性肾功能不全组抗β1和M2受体抗体阳性率分别为56.7%和38.1%,明显高于高血压组(18.3%和11.7%)和对照组(17.5%和15.0%)(P<0.01)。慢性肾功能不全亚组抗β1和M2受体自身抗体阳性率分析,尿毒症早期和尿毒症中期明显高于终末期尿毒症组,3组间差异有统计学意义(P<0.001)。结论抗β1和M2受体自身抗体可能与慢性肾功能不全发病有关,同时与肾损害程度有关。

Objective To explore the roles of autoantibodies against β1 adrenoceptor （β1-receptor） and M2 cholinergic receptor （M2-receptor） in patients with chronic renal insufficiency. Methods The epitopes of the second,extracellular loop of β1 receptor and M2 receptor were synthesized and used respectively to detect the sera autoantibodies against β1 receptor and M2 receptor by enzynle linked immunosorbent assay （ELISA） in 76 patients with chronic renal insufficiency, 60 cases with hypertension and 40 healthy controls. Results In patients with chronic renal insufficiency, the positive rates of the autoantibodies against β1-receptor and M2-receptor were 56.7% and 38. 1% respectively, which were much higher than those of patients with hypertension（ 18. 3% and 11.7% ） and higher than those of healthy controls （ 17.5% and 15.0% ） （all P 〈 0.01 ）. Conclusion The autoantibodies against β1 and M2-receptor may play important roles in the pathogenesis of chronic renal insufficiency.