槲皮素-3-O-芹菜糖基芦丁糖苷对新生大鼠海马神经前体细胞增殖的影响

Effect of [quercetin 3-O-apiosy1(1→2)]-rhamnosy1(1→6)-glucoside on the proliferation of neural progenitor cells from neonatal rat hippocampus

目的探讨一种新型抗抑郁剂槲皮素-3-O-芹菜糖基芦丁糖苷（代号：CTN-986）对神经前体细胞增殖的影响。方法分离培养新生大鼠海马神经前体细胞，运用免疫细胞化学的方法对所培养的细胞特性进行鉴定。用MTT法和^3H-胸腺嘧啶核苷参入法分别观察CTN0986对新生大鼠海马神经前体细胞增殖的影响，并初步探讨其作用机制。结果所培养的细胞具有神经前体细胞的特性，药物研究发现，氟西汀和CTN-986（2～250μmol·L^-1）作用4d，可以促进神经前体细胞的增殖；同时给予5-HT1A、受体特异性拮抗剂WAY-100635（0．1μmol·L-1）可以对抗CTN-986诱导的促神经前体细胞的增殖。结论CTN-986可以促进海马神经前体细胞的增殖，并且该作用的发挥与激活5-HT1A受体关系密切。

Aim To study the mechanism of a novel antidepressant [ quercetin 3-O-apiosyl （ 1→2 ） ] -rhamnosyl （ 1→2 ） -glucoside（ CTN-986 ） on the proliferation of hippocampal neural progenitor cells. Methods We isolated and cultured neural progenitor cells, and identified the cells with immunocytochemistry method. By using MTY assay and ^3H-thymidine incorporation assay, the effect of CTN-986 on neural progenitor cells proliferation was observed. Results The ceils we cultured possessed the characteristics of neural progenitor cells. When exposed to fluoxetine or CTN-986 （2250 μmol·L^-1 ） for 4 d, the proliferation of hippocampus neural progenitor cells was increased significantly. In the presence of WAY-100635 （0. 1 μmol ·L^-1 ）, CTN-986 induced cells proliferation was attenuated. Conclusion CTN-986 increases the proliferation of hippocampal neural progenitor cells which is likely dependant on 5-HTIA receptor.