高分子量聚乙烯对成骨细胞活性及新骨形成的影响

Influence of High Molecular Weight Polyethylene on Viability of Osteoblasts and New Bone Formation

研究高分子量聚乙烯(H igh m o lecu lar w e igh t po lyethy lene,HMW P)材料对成骨细胞活性及其在骨折愈合过程中对新骨形成的影响。(1)体外实验:在HMW P浸提液和标准培养基中培养成人骨髓源成骨细胞,采用M TT法检测成骨细胞的活性、碱性磷酸酶试剂盒检测成骨细胞的生理功能。(2)体内实验:制作犬股骨远端粉碎性骨折模型,用HMW P板与钢板互锁组合固定,术后3、6、9、12周取材,分别进行HE染色组织学观察及扫描电镜观察HMW P板下骨折端的新骨生成情况。结果发现:(1)采用HMW P浸提液和标准培养基培养的2组成骨细胞,在第2、4、8、14 d的增殖能力和碱性磷酸酶活性无统计学差异,P>0.05;(2)术后不同时间点进行肉眼观察、HE染色组织学观察及扫描电镜观察均可见HMW P板与骨组织之间界面无吸收,HMW P下骨折端的新生骨组织随着时间延长不断成熟。提示:HMW P对成骨细胞的活性及新骨形成均无不良影响,可作为内固定材料应用于骨折的治疗。

To investigate the influence of high molecular weight polyethylene （HMWP） on the viability of osteoblasts and new bone formation in the process of fracture healing,the osteoblasts derived from adult human bone marrow were cultured in HMWP maceration extract and normal culture medium. The viability of the osteoblasts was measured by MTT assay, and the function of the osteoblasts was detected by use of alkaline phosphatase test kit. The locked double-plating （steel plate and HMWP plate） was implanted and fixed at the artificial fracture of distal femur of dogs. Specimens were gained at 3, 6, 9 and 12 weeks postoperatively, examined with macroscopy, microscope and scanning electron microscope （SEM）. The results showed that HMWP did no harm to osteoblasts. There is no significant difference in activities of proliferation and alkaline phosphatase between HMWP maceration extract and normal culture medium at each observation time of at 2,4,8, and 14 dyas （P〉0.05）. Bone tissue under the implanted HMWP plate manifested no absorption; the new bones formed under the HMWP plate and gradually matured as time went on. It is demonstrated in this study that HMWP has no adverse influence on the viability of osteoblasts and new bone formation and it can be used as internal fixation implant in treating fractures.