胆管癌微血管生成及其病理学特征与预后的关系

The relationship between angiogenesis of cholangiocarcinoma and clinical pathology and prognosis

目的探讨胆管癌微血管生成及其病理学特征与预后的关系。方法应用免疫组化法检测50例胆管癌标本的微血管密度、血管内皮生长因子和血管内皮生长因子受体Flk-1／KDR的表达,结合临床病理学资料和随访资料进行分析。结果胆管癌组织和癌旁组织的微血管密度 (34．04±11．08、32．80±9．28)均高于正常组织(11．67±4．64)(P<0．01);血管内皮生长因子和 Flk-1／KDR在肿瘤组织和癌旁组织中的表达均高于正常组织(P<0．01);伴淋巴结转移组微血管密度 (41．07±11．83)高于无转移组(30．93±9．18)(P<0．05);伴神经浸润组微血管密度(37．85±12．04) 高于无浸润组(30．32±8．51)(P<0．05);微血管密度<30和30-39组的生存率高于≥40组(P< 0．05)。结论微血管生成与胆管癌的发生、发展和预后密切相关。

Objective To study the relationship between angiogenesis and pathology and prognosis of cholangiocarcinoma. Methods lmmunohistochemistry was used to determine micro-vessel density （MVD） and the expression of vascular endothelial growth factor（VEGF） and its receptor FIk-1/KDR in 50 eholangiocarcinoma cases. Results were compared with pathological and follow-up parameters. Results MVD in cholangiocarcinoma tissues and para-tumor tissues （34.04 ± 11.08, 32.80 ± 9.28） were higher than normal bile duct tissues （ 11.67 ± 4. 64） （ P 〈 0. 01 ）. Expression of VEGF and EIk-1/KDR in cholangiocarcinoma tissues and para-tumor tissues were significantly higher than those in normal bile tissues： MVD of the eases with lymph node metastasis or nerve invasion（41.07 ± 11.83, 37.85 ± 12.04） were higher than negative cases（ 30.93 ± 9. 18, 30.32 ± 8.51 ） （P 〈 0.05 ）. Survival rate of cases with M VD 〈 30 and between 30 - 39 were higher than those with MVD ≥ 40 （ P 〈 0. 05 ）. Conclusions There was significant relationship between tumor angiogenesis and poor prognosis in cases of cholangiocarcinoma.