拟南芥肌动蛋白解聚因子4基因(AtADF4)在烟草中表达引起植株形态变化

Expression of an Arabidopsis Actin-Depolymerizing Factor 4 Gene (AtADF4) in Tobacco Causes Morphological Change of Plants

用PCR方法从拟南芥基因组DNA中分离克隆肌动蛋白解聚因子基因（AtADF4），并进行了序列分析。通过农杆菌介导转化将AtADF4导入烟草，PCR和RT—PCR检测证明AtADF4已整合到烟草基因组中并得到表达。转基因烟草幼苗下胚轴的弯曲度在暗培养与光照培养条件下均比对照的大，暗培养条件下下胚轴弯曲程度较高；下胚轴薄壁细胞壁比对照大，维管束排列不整齐；根毛稀少弯曲，而对照根毛密集且直；转基因烟草开花时间比对照平均延迟了7～8d，花粉萌发时花粉管比对照粗短。

The actin-depolymerizing factor 4 gene （ADF4） of Arabidopsis thaliana was cloned and sequenced （Figs. 1, 2）. The plant expression vector with ADF4 was constructed and transformed into tobacco by Agrobacterium tumefaciens. Molecular identification showed that the ADF4 gene was integrated into the genome of tobacco and expressed in transgenic tobaccos assayed by PCR and RT-PCR （Fig.3）. Expression of an Arabidopsis thaliana ADF4 gene in tobacco resulted in morphological change of plants. The effects of ADF4 on transgenic tobaccos growth were as follows： the hypocotyls of transgenic plants were wavy, especially in darkness, whereas those of the control were straight （Fig.4A）; the root hairs of transgenic plants were less than the control, and they were also wavy （Fig.4B）; the parenchyma cells of transgenic plants were larger than the control and the arrangement of vascular bundle was out of order （Fig.4C）; the flowering time of T2 line was at least 7 days later than the control; the pollen tubes of transgenic plants were shorter than those of the control （Fig.4D）.