阿托伐他汀钙对肾血管性高血压大鼠模型左室重构的影响

Effects of atorvastatin on cardiovascular remodeling in renovascular hypertensive rats

目的观察阿托伐他汀钙对肾血管性高血压大鼠左室重构的保护作用。方法应用经典的两肾一夹方法,制备肾血管性高血压大鼠模型。实验动物随机分对照组(假手术)、高血压组及治疗组,每组各7只。治疗组给予阿托伐他汀钙(立普妥2 mg/kg)治疗6周。采用鼠尾测压法测定大鼠血压的变化;放射免疫分析法测定大鼠血浆血管紧张素(Ang)Ⅱ含量和肾素活性;称量法计算各组大鼠心脏重量及左室重量与体重比值。结果高血压大鼠血浆AngⅡ含量和肾素活性分别为(106.4±7.8)ng/L和(20.6±2.4)ng/L,比对照组〔(72.3±5.4)ng/L和(12.5±3.7)ng/L〕明显增高(P<0.01);心脏重量为(1.46±0.09)g,左室重量与体重比值为(3.54±0.19)×10-3,比对照组〔(0.98±0.07)g和(2.28±0.06)×10-3〕显著增大(P<0.01)。给予小剂量阿托伐他汀钙治疗6周后,AngⅡ含量〔(68.3±6.9)ng/L〕和肾素活性〔(8.7±2.3)ng/L〕明显下降(P<0.01),心脏重量减至(1.05±0.04)g,左室重量与体重比值降至(2.36±0.07)×10-3,与对照组比较差异无统计学意义(P>0.05)。结论阿托伐他汀钙能明显降低高血压大鼠心脏重量及左室重量与体重比值,对高血压大鼠病理性左室重构具有保护作用。

Objective To investigate the effects of atorvastatin on cardiovascular remodeling in renovascular hypertensive rats. Methods The 2-kindey,1-clip hypertensive rats（2K1C, Goldblatt） were prepared with Sprague-Dawley rat. SD rats were randomly divided into three groups： normal control rats, hypertensive rats and hypertensive rats treated with atorvastatin （2 mg · kg^-1 · d^-1） . After 6 weeks treatment, systolic blood pressure（SBP） was measured using the tail-cuff method. The plasma concentration of angiotensin Ⅱ and renin activity were determined by radioimmunoassay. The heart weight, the ratio of left ventricular weight to body weight were calculated. Results The plasma concentration of angiotensin Ⅱ [ （106.4±7.8） ng/L] and renin activity [ （20.6 ± 2.4） ng/L] were significantly increaed in hypertensive rats compared with normal rats（（72.3±5.4） ng/L and （12.5±3.7） ng/L）（P（0.01）. The heart weight（（1.46±0.09）g）, the ratio（3.54±0.19（×10^-3）） of left ventricular weight to body weight in hypertensive rats were obviously higher than those of normal rats（（0.98± 0.07）. g and （2.28 ± 0.06）×10^-3） （P〈0.01） . After treatment with atorvastatin, the plasma concentration of angiotensin Ⅱ [（68. 3±6. 9） ng/L]and renin activity[（8.7± 2.3 ）ng/L）, heart weight[（1.05±0.04）g], the above ratio（2.36±0.07（×10^-3）]were decreased significantly,there were no significant differences between the groups of hypertensive rats and the normal. Conclusions Atorvastatin can decrease the ratio of left ventricular weight to body weight and has protective effects against cardiovascular remodeling in hypertensive rats.