skp2,C-myc在肝细胞肝癌中的表达及其意义

Expression and significance of skp2 and C-myc protein in hepatocellular cancinoma

目的研究skp 2和C-myc在肝细胞肝癌组织中的表达及其与临床病理指标的关系。方法用免疫组化PV 9 0 0 0二步法检测skp 2和C-myc蛋白在4 8例肝细胞肝癌、2 0例肝硬化患者和1 6例正常肝组织中的表达。结果肝细胞肝癌组织中skp 2的阳性表达率(3 3.3%)显著高于肝硬化组织(均为阴性表达)(P=0.0 0 8)和正常肝组织(均为阴性表达)(P=0.0 2 0)。skp 2在肝细胞肝癌中的表达与组织分化程度及转移有关(P<0.0 0 1及P=0.0 1 7),但与肿瘤大小无关(P=0.058),skp 2在高分化肝细胞肝癌中无表达。肝细胞癌组织中C-myc蛋白的的阳性表达率为5 8.3%,显著高于肝硬化组织的1 5%(P=0.0 0 1)和正常肝组织(阴性表达)(P<0.0 0 1)。C-myc蛋白阳性表达与组织分化程度、转移及肿瘤大小有关,组间差异依次为P<0.0 0 1,=0.0 2 3及=0.0 0 7。肝细胞癌中skp 2蛋白的表达与C-myc的表达呈正相关(r=0.5 0 8,P<0.0 0 1)。结论C-myc可能与肝细胞肝癌的发生发展有关。Skp 2的表达提示预后不良。C-myc可能对skp 2的表达起正性调节作用。

Objective To investigate the expression of Skp2 and C-myc in hepatocellular carcinoma and its relationship with clinicopathologic characteristics. Methods The expression of skp2 and C-myc was evaluated in hepatocellular carcinoma tissues of 48 patients, liver cirrhosis tissues of 20 cases , and normal liver tissues of 16 patients by immunohistochemistry （ PV9000 two steps methods ） . Results The expression rate of skp2 （33.3%） in hepatocellular carcinoma tissues was significantly higher than that in liver cirrhosis tissues （ negative expression ） （ P = 0. 008 ） and normal liver tissues （ negative expression ） （ P = 0. 020 ） . The expression of skp2 was correlated with histological differentiation and metastasis （ P 〈 0. 001 and P = 0.017 ） , but not with tumour size （ P = 0. 058 ） , and it was not expressed in highly differentiated hepatocellular carcinomas. The expression rate of C-myc protein in hepatocellular carcinoma tissues （58.3 % ） was significantly higher than that in liver cirrhosis tissues （ 15 % ） （ P ： 0.001 ） and normal tissues （ negativ e expression ） （ P 〈 0.001 ） . The expression of C - myc was correlated with histological differentiation （ P 〈 0.001） , metastasis （ P = 0. 023 ） , and tumour size （ P = 0. 007 ）. The expression of skp2 in hepatocellular carcinoma was positively correlated with the expression of C-myc（ r = 0. 508, P 〈 0. 001 ）. Conclusions C-myc gene may be closely related to the occurrence and development of hepatocellular carcinoma. The expression of skp2 indicates a poor prognosis. The expression of skp2 in hepatocellular carcinoma may be positively modulated by the activation of C- myc gene.