摘要
目的:检测脑源性神经营养因子(BDNF)干预急性高眼压后大鼠视网膜 Müller 细胞谷氨酰胺合成酶(GS) 和谷氨酸/天冬氨酸转运体(GLAST)表达的变化,探讨 BDNF 保护节细胞(RGCs)的可能机制。方法:成年大鼠分为3个 BDNF 干预组和3个溶媒对照组并进行玻璃体内注射。2 d 后将预处理动物左眼眼压升高至闪光视网膜电图 b 波消失的临界眼压且维持缺血60 min。实验动物分别存活1、3或7 d 后通过免疫组织化学检测大鼠视网膜 GS 和 GLAST 的表达变化。结果:与正常对照组比较,溶媒对照组 GS 和 GLAST 在存活1 d 和3 d 时表达上调,7d 时下降;BDNF 干预组未出现表达明显变化。结论:BDNF 可能不是通过 Müller 细胞上调 GS 和 GLAST,降低胞外 Glu 来保护 RGCs。
Objective: To investigate the protective mechanism Of brain-derived neurotrophic factor (BDNF) on retinal ganglion cells (RGCs) through detecting expression of glutamine synthesase (GS)and glutamate/aspartate transporter (GLAST) of Mueller cells in BDNF pre-treated acute hyper-intraocular pressure rat retina. Methods: Left eyes of rats were divided into 3 BDNF pre-treated groups and 3 vehicle control groups (received intravitreal injection; the right eyes served as control. The intraocular pressure of the BDNF pre-treated (2-day) eyes was increased to make b wave of flash electroretinogragh (FERG) disappear and maintained for 60 min. 1,3 and 7days later, expression of GS and GLAST of rat retina were detected using immunohistochemistry. Resulst: Compared with normal control groups, expression of GS and GLAST of vehicle control groups were increased after 1 and 3 days and decreased gradually, while that of BDNF pre-treated groups had no remarkable changes. Conclusion: It seems that BDNF's protection effect on RGCs is not through up-regulating GS and GLAST in Mueller cells and decreasing extracelluar Glutamate (Glu).
出处
《解剖学杂志》
CAS
CSCD
北大核心
2006年第1期14-17,109,共5页
Chinese Journal of Anatomy
基金
国家自然科学基金(30100098)湖南省自然科学基金(00JJY20105)