氮分子激光照射及激光加光敏剂8-MOP照射对白色念珠菌生长的影响

The effects of nitrogen laser irradiation and psoralen phototoxicity on the growth of Candida albicans

实验研究了盘激光照射及激光加光敏剂8—MOP对白色念珠菌生长的影响。低能量氮激光照射(小于0.6mJ/cm^2)30′—60′未见明显影响。如外加8—MOP时对霉菌生长发生中等抑制作用,但单独应用8—MOP则未见抑制作用。因此,这种抑制是由于光敏化作用引起的。较高能量氮激光照射(1.0mJ/cm^2)15′—90′时,不论单独照射或外加8—MOP,皆使霉菌生长完全抑制。作者认为,达到一定能量的氮激光照射可完全抑制白色念珠菌生长,氮激光似可临床应用于光照疗法及光化学疗法。

The effects of nitrogen laser irradiation and psoralen phototoxicity on the growth of C. albicans were examined in vitro. Nitrogen laser irradiation at low energy （less than 0.6mJ/sq cm） for 30′t ： 90′ did not oause any significant effect on the growth of C. albicans. Such irradiation in the presence of 8-MOP rosalted in a moderate inhibitory effect on fungal growth. 8- MOP application alone did not show such an etteet. Thus the etteet on fungal cells was a photoseusitization. At higher laser deasity（1. 0 mJ/sq cm）. exposure of fungal suspensions in the presence or absence of 8-MOP for 15′ to 90′ resulted in a marked inhibitory or iethal effect on the fungus as measured by Ioss of colony forming ability. It seams that at certain laser densities, nitrogen laser trradiation alone is sufficient to inhibit fungal growth completely. These observations suggest possible clinical application of nitrogen laset in modern phototherapy and photochemotherapy.