蒙药阿如健脾散质量标准研究

Study on Quality Standard of Mongolian Medicine Aru Spleen-invigorating Powder

目的:对阿如健脾散中的荜茇、丁香、诃子等成分进行薄层鉴别,同时测定了该制剂中荜茇的有效成分胡椒碱的含量.方法:HPLC采用C18柱,甲醇-水(77∶23)为流动相,流速0.5 ml/min,检测器波长343 nm;TLC采用阴阳对照实验,对方中3种药材进行了确认,已达到鉴别的目的.结果:3种药材被鉴别;采用HPLC测定胡椒碱的含量,在0.02～0.10 μg范围内与峰面积有良好的线性关系(r=0.9997),平均加样回收率为97.02%,RSD为1.03%.结论:所用方法简便、稳定,能有效的控制该制剂的质量.

Objective：To establish a TLC method for the identification of long pepper, caryophyllus, chebule as well as an HPLC method for determination of piperine as an effective component in Aru Spleen -invigorating powder. Methods： HPLC method was carried on C18 column using methanol-water （77：23）as mobile phase. Detective wavelength was set at 343nm. Results：The three medicines contained in the preparation were identified by TLC with control test. In HPLC method, the calibration curve for gallic acid was linear in the range of 0.02-0.10μg. The average recovery was 97.02 % with RSD=1.03 %. Conclusion：This method is simple, rapid and reliable for quality control of this preparation.