摘要
白血病抑制因子(leukemiainhibitorfactor ,LIF)可以抑制小鼠胚胎干细胞分化,维持其自我更新,因而用于该干细胞的体外扩增。由于不同的胚胎或成体干细胞分化特性相差很大,因此,LIF可能对不同的干细胞有不同的作用。对新鲜分离的小鼠外胚间充质细胞(ectomesenchymalcells ,EMCs)在有或无LIF的培养条件下,观察了细胞生长的特性,表型变化等,并通过检测其中外胚间充质干细胞(ectomesenchymalstemcells ,EMSCs)的水平,探讨LIF与EMSCs的关系。结果表明:(1)LIF的受体gp130表达于未分化与早期分化的EM SCs表面,提示LIF对于EMSCs具有潜在的调节作用;(2 )LIF促进小鼠EMSCs的自我维持,bFGF则对此具有协同作用。因此,LIF与bFGF联合对体外培养和扩增小鼠外胚间充质干细胞,以及对其深入研究和应用具有重要意义。
Leukemia inhibit factor（LIF） has been found a valuable differentiation- inhibiting factor for maintaining mouse embryonic stem cells in vitro. However, whether this cytokine similarly effects on other stem cells in body is still unknown. In present study, primary ectomesenchymal cells（EMCs） are isolated from E12.5 BALB/c mice, cultured in DMEM/F12 standard medium or mediums supplemented with 1000U/ml LIF, 20ng/ml bFGF, or LIF ＋ bFGF. Using p75 as a marker, the percentage of ectomesenchymal stem cells （EMSCs） in EMCs is assayed. Two main findings emerged from these analyses： （1） gp130 （LIF receptor） expressed on EMSCs surface, suggestting LIF has potential function on EMSCs. （2）LIF can obviously inhibit EMSCs to differentiate, maintain EMSCs survive in vitro, and such effection can be enanced by cooperation of bFGF. These results suggest that LIF also is a differentiation - inhibiting factor for mouse EMSCs. Thus, LIF and bFGF can be used for amplifying such stem cells in vitro.
出处
《生物医学工程研究》
2005年第1期28-31,共4页
Journal Of Biomedical Engineering Research
基金
第四军医大学创新工程基金重点课题 (CX0 2F0 0 2 )。
