转基因抗虫白菜对菜青虫、小菜蛾抗性的研究

Studies on Resistance against Common Cabbage Worm (Pieris rapae Linnaenus) and Diamond Moth(Plutella xylostella Linnaenus) of Transgenic Insect-resistant Cabbage

对导入马铃薯蛋白酶抑制剂基因(pinⅡ)的大白菜和菜心植株当代和自交后代进行了菜青虫(Pieris rapae L.)和小菜蛾(Plutella xylostella L.)的抗性筛选.室内生物测定表明:取食转基因植株的1,2龄幼虫死亡率显著高于对照;取食转基因T1大白菜的菜青虫生长发育受到明显抑制,转化株老叶对菜青虫幼虫的毒杀作用明显高于嫩叶;取食转基因T2大白菜和菜心不同转化株的小菜蛾生长发育受到不同程度的抑制,转基因大白菜比菜心对小菜蛾有更好的抗虫效果,取食转基因菜心2～24植株小菜蛾的幼虫死亡率最高达64%,取食转基因大白菜2～6小菜蛾幼虫死亡率最高达90%;取食转基因植株小菜蛾的化蛹率、蛹重和羽化率亦有不同程度的降低.

In vitro leaf tests of T1 and T2 plants from pin Ⅱ - transgenic Chinese cabbage and flowering Chinese cabbage for evaluating resistance to Common Cabbage Worm （ Pieris rapae Linnaenus） and Diamond Moth （ PluteUa la Linnaenus） larvae were made under laboratory condition. The results showed that larvae fed with transgenic leaf tissue had a higher mortality especially in 1 st and 2nd instar. The test of common cabbage worm larvae fed with T1 transgenic Chinese cabbage revealed that the developments were retarded, and the mortality of larvae fed with old leaves was signifi- cantly higher than that of larvae fed with young leaves. The test of diamond moth larvae fed with T2 transgenic Chinese cabbage and flowering Chinese cabbage revealed that the developments were retarded in varying degree; The transgenic Chinese cabbage produced more effective insect - resistance than transgenic flowering Chinese cabbage, the mortality of larvae fed with flowering Chinese cabbage strain 2 - 24 was 64 % and that of larvae fed with Chinese cabbage strain 2 - 6 was 90 % ; The weight of pupae, percent of pupation and percent of eclosion were lower than non - transgenic plants in different degree.