人血栓调节蛋白在中国仓鼠卵巢细胞中的表达与鉴定

The Expression and Identification of Human Thrombomodulin in Chinese Hamster Ovary Cells

目的 获得表达人血栓调节蛋白的CHO-TM细胞，以便研制临床诊断治疗制品。方法 重组表达质粒pTh402提纯后利用lipofectamine脂质体转染中国仓鼠卵巢细胞（CHO）．C418筛选后对阳性细胞用蛋白质印迹术（Westem blorting）定性检测人血栓调节蛋白（hTM）的表达情况。结果 血栓调节蛋白成功导入宿主细胞CHO并获得高效表达。结论 获得高效表达hTM的细胞CHO-TM，为进一步研究临床诊断治疗制荆创造了条件。

Objective To obtain human thrombomodulin（hTM） expression in Chinese hamster ovary cells （ CHO） . Methods Recombinant expression plasmld pThr402 was tranffected into CHO by lipofeetamine and obtained expression after selecting with G418. hTM expression in CHO was identified by Western blotting. Results TM was transfected into CHO successfully and obtained expression. Conclusions CHOTM which can efficiently express human thrombomodulin is obtained. It provides a base for clinic diagnosis and therapy.