宫颈腺癌中FHIT蛋白表达与HPV感染的研究

Study of the Expression of FHIT in Cervical Adenocarcinoma and the Relation to HPV Infection

目的探讨脆性组氨酸三联体(FHIT)基因在宫颈腺癌发生发展中的作用,分析FHIT蛋白表达与人乳头瘤病毒(HPV)感染的关系。方法采用组织微阵列技术结合免疫组化(二步法)检测FHIT蛋白与HPV16/18-E6蛋白在86例宫颈腺癌组织和24例慢性宫颈炎组织中的表达。结果FHIT蛋白在宫颈腺癌组织中的阳性表达率为46.5%,较慢性宫颈炎组织100.0%明显降低(P<0.01);HPV16/18-E6蛋白阳性表达率为65.1%,明显高于慢性宫颈炎组织4.2%(P<0.01)。FHIT蛋白表达与宫颈腺癌病理分级和组织学类型有关,G2、G3组FHIT阳性表达率为39.4%和22.7%,均明显低于G1组(P<0.05),透明细胞腺癌FHIT阳性表达率为22.2%,明显低于其它类型宫颈腺癌(P<0.05)。HPV16/18-E6蛋白表达与宫颈腺癌病理分级和组织学类型无关(P>0.05)。结论宫颈腺癌的发生发展与HPV感染及FHIT表达缺失密切相关。

Objective To study the rote of fragile histidine triad （FH1T） gene in occurrence and devetopment ot cervical adenocarcinoma,and to analyse the rdationship between the expression of FHIT protein and human papilloma virus （HPV） infection. Methods The expressions of FHIT and HPVI6/18-E6 proteins were determined by tissue microarray combined with immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammationl Results The positive rate of FHIT in cervical adenocarcinoma was 46,5% ,which significantly lower than that in cervical chronic inflammation 100.0% （P 〈 0.01）. The positive rate of HPV16/18-E6 protein in cervical adenocarcinoma was 65.1% ,which significantly higher than that in cervical chronic inflammation 4.2%（P 〈 0.01）. In cervical adenocareinoma the loss of FHIT expression was significantly related to pathological grades and histological types. The positive rates of FHIT in G2 and Ga are 39.4% and 22.7% ,which significantly lower than in G1（P 〈0.05）. The level of FHIT positive expression in clear-call adenocarcinoma is 22.2% ,which significantly lower than in other types （P 〈 0.05）. The expression of HPV16/18-E6 protein was correlated neither with pathological grades nor histological types（ P 〉 0.05）. Conclusion Carcinogenesis and devdopment of cervical adenocareinoma are dosdy related to the loss of FHIT expression and HPV infection.