中成药中非法添加利巴韦林检测方法的研究

Study on the Detection Method of Ribavirin Illegally Added in Antiviral Chinese Patent Medicine

为建立中成药中检测非法添加利巴韦林的定性定量方法，采用薄层色谱、质谱法定性，高效液相色谱法定量．色谱柱为Shim-pack VP-ODS柱（250mm×4．6mm。5μm）．流动相为甲醇-水（8：2）·检测波长（λ）为206nm．峰面积外标法定量。结果在线性范围为10-250μg／ml．r=0．9992；平均回收率为98．45％，相对标准偏差为1.53％。说明根据标准品、样品利巴韦林的比移值大小和碎片离子峰的特征．能够很好地定性。采用高效液相色谱法能够快速分离，准确定量。

To establish a method for detecting ribavirin illegally added in antiviral Chinese patent medicine, TLC and MS were used for the qualitative analysis, and RP-HPLC was used for the quantitative analysis. A Shim-pack VP-ODS column （250 min× 4. 6 mm, 5μm） was used with methanol-water （8 ： 2 ） as the mobile phase, the detection wavelength was 206nm, peak area external reference method was used. The linear range of 10-250μg·ml^-1 （r=0. 9992） and an average recovery of 98.45% ,RSD=1.53% （n=7） were obtained. Ribavirin can be qualitative analyzed well according to the Rfvalue and feature of fragment ion peak of ribavirin in standard preparation and samples, and it can be separated quickly with other composition and quantitative analyzed accurately by HPLC.