摘要
本文提出了一种简单易行的测定微生物中胆碱脱氢酶活力的方法。通过测定549nm波长下电子传递体系PMS(吩嗪二甲酯硫酸盐)-cty.c(细胞色素C)光吸收值的增加,就可以计算出胆碱脱氢酶活力。利用此方法测定了大肠杆菌胆碱脱氢酶热激诱导前后的活力,结果发现受到诱导的大肠杆菌中胆碱脱氢酶的活力明显高于对照组。
This paper investigated a simple and available method for measuring the enzyme activity of Choline dehydrogenase（CDH） in microorganisms, it can be used as a parameter to estimate the synthesis of betaine in organisms. The enzyme activity of CDH can be calculated by measuring the absorption of cty. c at 549nm in electron transportation system PMS-cty. c. The result showed that the enzyme activity of the induced E. coli was higher than those of the control group＇ s obviously.
出处
《山东科学》
CAS
2006年第1期32-34,49,共4页
Shandong Science
