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高效液相色谱法测定3-甲基吲哚的含量 被引量:1

Determination of 3-methylindole by High Performance Liquid Chromatography
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摘要 采用Hypersill BDS C18色谱柱,以甲醇-水(85+15)为流动相,流速1.0mL.min^-1,254nm为检测波长,建立测定3-甲基口9I哚的高效液相色谱法。进样量在(2~14)ug范围内线性关系r=0.9996,回收率在98.9%~99.9%,RSD不大于0.49%。 A HPLC method was established for the determination of 3 - methylindole, which consisted of a Hypersill 8DS C18 column (4.6 mm× 200 mm, 5um) and methanol - water ( 85 : 15 v/v) mixture as mobile phase, detected at 254 nm with flow rate of 1.0 mL/min. The linear range was 2 - 14 ug ( r = 0. 9996) and the average recovery was 98.9 ~ 99.9%, with RSD of 0.49%.
作者 丁玲玲 臧娜
出处 《云南化工》 CAS 2006年第1期49-50,共2页 Yunnan Chemical Technology
关键词 高效液相色谱法 3-甲基吲哚 HPLC 3 - methlindole analysis
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