摘要
采用Hypersill BDS C18色谱柱,以甲醇-水(85+15)为流动相,流速1.0mL.min^-1,254nm为检测波长,建立测定3-甲基口9I哚的高效液相色谱法。进样量在(2~14)ug范围内线性关系r=0.9996,回收率在98.9%~99.9%,RSD不大于0.49%。
A HPLC method was established for the determination of 3 - methylindole, which consisted of a Hypersill 8DS C18 column (4.6 mm× 200 mm, 5um) and methanol - water ( 85 : 15 v/v) mixture as mobile phase, detected at 254 nm with flow rate of 1.0 mL/min. The linear range was 2 - 14 ug ( r = 0. 9996) and the average recovery was 98.9 ~ 99.9%, with RSD of 0.49%.
出处
《云南化工》
CAS
2006年第1期49-50,共2页
Yunnan Chemical Technology