白皮松胚性愈伤组织诱导因素的研究

Factors Influencing the Initiation of Embryogenic Callus in Pinus bungeana

以不同发育时期的未成熟合子胚为外植体,对白皮松胚性愈伤组织诱导因素进行了研究。结果表明,DCR 1培养基为白皮松胚性愈伤组织诱导的最适培养基;6 m g.L-12,4-D和2 m g.L-16-BA是最优激素组合,诱导率高达97.22%;最佳采样时期为6月30日前后。

Embryogenic callus was induced from different growth periods of immature embryos of Pinus bungeana. Factors affecting initiation were investigated. The results showed that DCR1 was an optimal medium for iniation. The optimal combination of hormone was 6 mg · L^-1 2,4-D and 2 mg · L^-1 6-BA. The inducing rate was 97. 22%. The best period of immature zygotic embryo sampling was around June 30.