摘要
研究了氧化亚铁钩端螺菌(Leptospirillums ferrooxidans)发酵罐补料连续培养的最佳条件。结果表明:pH为1.2,接种量为33%(V/V),充气量为6 L/min,搅拌转速为450 r/min时,培养30 h后,用3 mL/min的速度补料,补料1 500 mL后,菌密度可达1.0×1010个/mL,高于文献报道。
The optimum conditions of the fed-batch technology on Leptospirillums ferrooxidans are studied. The results show that the optimum fermentation conditions are as follows： pH is 1.2, inoculation quantity is 33%, air flow is 6 L/min, the rotational speed is 450 r/min. After 30 h culture time the material is added at a speed of 3 mL/min. After 1 500 mL, the bacteria density reaches 1.0×10^10/mL, which is higher than the number reported by the past articles.
出处
《铀矿冶》
CAS
北大核心
2006年第1期43-46,共4页
Uranium Mining and Metallurgy
基金
东华理工学院硕博启动基金项目(DSH0510)
关键词
氧化亚铁钩端螺菌
高密度
补料法
Leptospirillums ferrooxidans
high density
fed-batch fermentation
