宽体金线蛭嗜水气单胞菌感染的病原检验

Examination of A.hydrophila Isolated from Whitmania pigra (L.)

对河北某宽体金线蛭(Whitmania pigraW h itm an)养殖场所养殖的宽体金线蛭发生的病害,进行了发病情况、临床表现、病理变化等方面的检验。同时,择代表菌株进行了16S rRNA基因的分子鉴定,测定了16S rRNA基因序列、分析了相关细菌相应序列的同源性、构建了系统发生树。结果表明所检病例为由嗜水气单胞菌(Aerom onas hydrophila)所引起的感染。分离后做纯培养的10株嗜水气单胞菌均为同种血清型菌株;代表菌株对健康宽体金线蛭的人工感染试验表明了相应的原发病原学意义;药敏试验结果显示,对供试37种抗菌药物中的头孢噻肟等高度敏感、对链霉素等敏感、对苯唑青霉素等耐药。

We examined diseases occurring farmed Whitmania pigra （ L. ） in Hebei province in the aspect of situation of disease, clinical symptoms and pathological changes. In addition, the molecular identification were conducted to representative strain, the 16S rRNA gene was sequenced and compared with that of related strains, molecular phylogenctic tree was constructed. The results showed that the disease was infected by Aeromonas hydrophila, Pure cultures of 10 strains have the same serotype, Selected representative strain was proved to be the corresponding primitive causal agent of the disease by artificial infection experiment to healthy Whitmania pigra （ L. ） . Antibiotic sensitivity of the isolates to used thirty-seven antimicrobial agents showed that the tested strains were high sensitive to cefotaxime et al. , were sensitive to streptomycin et al. , were resistant to oxacillin et al.