在无有机碳源和缺氧条件下自养菌和异养菌脱氨氮作用

Removal of NH_3-N by Microbe Without Organic Carbon Source under Oxygen Limited Condition

在无有机碳源和缺氧条件下,应用15N示踪考察4株具有氨氧化作用的菌株在膜反应器中的氨氧化产气情况。当溶氧浓度(DO)<0.5mg/L的缺氧条件下,自养菌Nitro-som onassp.单株培养,能将6.3%的氨氮转化为氮气,15N2产生量占氨氮消耗量的21.86%。自养菌株与异养菌株混合培养,可将30.86%的氨氮转化为氮气,15N2产生量占氨氮消耗量的80.38%。在无有机碳的条件下,自养菌和异养菌的协同代谢作用,可大大提高缺氧氨氧化产氮气的效率。

It was investigated by ^15N tracer that four strains capable of ammonia oxidation under the condition ot limited dissolved oxygen （DO） and without organic carbon source in a sealed bio membrane reactor. Each strain was cultured at room temperature, a sealed vessel filled with argon and an appropriate quantity of oxygen. When the condition kept DO 〈0.5mg/L, 6. 3% of ^15NH4^＋ - ^15N could be converted into ^15N2 by the single strain of Nitrosomonas sp. ^15N2 oecupied 21.86% of the quantity of consumed ^15NH4^＋ -^15N; whereas 30. 86% of ^15NH4^＋ - ^15N could be converted into di-nitrogen gas by mixed autotrophic and heterotrophic strains under the same condition, and ^15N2occupied 80.38% of the quantity of ^15NH4^＋- ^15N consumed. Cooperative metabolism of autotrophic and heterotrophic strains speeded up the transformation of NH4^＋ - N and production of N2 under DO 〈0. 5mg/ L condition without organic carbon source in the membrane reactor.