摘要
采用碳二亚胺法制备了氧氟沙星与牛血清白蛋白(BSA)及卵清蛋白(OVA)的结合物,通过FeCl3显色反应,紫外扫描以及动物免疫证明了人工抗原的成功合成。经紫外光谱法测定,每分子牛血清白蛋白连接的氧氟沙星分子数为11.16个;每分子卵清蛋白连接的氧氟沙星分子数为5.9个。
Carbodiimide method was employed in the synthesis of ofloxacin(OFL)with carriing proteins bovine serum albumin(BSA)and ovalbumin (OVA). qhe Conjugation of OFL-BSA and OFL-OVA was identified with FeCl3, UV Spectrum and immunizing animal. 11.16 ofloxacin molecules were linked to a molecule of the BSA, 5.9 ofloxacin molecules were linked to a molecule of the OVA.
出处
《安徽农业科学》
CAS
北大核心
2006年第3期487-488,602,共3页
Journal of Anhui Agricultural Sciences
基金
安徽省优秀青年科技基金(04041042)
关键词
氧氟沙星
碳二亚胺法
人工抗原
Ofloxaein
Carbodiimide method
Artificial antigen