大鼠骶髂关节传入神经通路的实验研究

Experimental study on afferent pathways of rat sacroiliac joint

目的:通过逆行神经追踪法研究大鼠骶髂关节的传入神经通路。方法:30只雄性Sprague-Dawley大鼠随机分成非交感神经切除组(A组)和交感神经切除组(B组),每组15只,交感神经切除组切除左侧L1以下椎旁交感干。两组左侧骶髂关节注入30%的辣根过氧化物酶(HRP)20μl,72h后取出双侧的L1-S1背根神经节(DRG),TMB法染色后在光学显微镜下观察HRP阳性神经元细胞并计数。结果:两组左侧L1、L2背根神经节内HRP阳性神经元差异有显著性意义(P<0·05),B组HRP阳性神经元明显减少;左侧L3-S1背根神经节内HRP阳性神经元差异无显著性意义(P>0·05)。结论:L1-S1神经节含有支配同侧骶髂关节的传入神经元,同侧椎旁交感干可能是骶髂关节到L1-L2神经节重要的传入神经旁路,而不是到L3-L5神经节的传入旁路或重要的传入旁路。

Objective： To investigate afferent pathways of rat sacroiliac joint with neural retro-tracing technique Methods： Thirty male Sprague-Dawley rats were randomly divided into sympathectomy group （group A,of which left paravertebral sympathetic trunk were cut down）and unsympathectomy group（group B）.20 μl 30% horseradish peroxidase （HRP） was injected into left sacroiliac joints in two groups. After 72 hours,bilateral L1 -S1 dorsal root ganglia （DRG） were harvested and processed by TMB method,in which HRP-positive neurons were observed and counted under light microscope. Results： There were significant differences in the number of HRP-positive neurons in left L1, L2 DRG between group A and group B （ P 〈 0.05）. HRP-positive neurons in group B obviously decrease than group A. However, there was no significant differences in left L3 - S1 DRG were observed between group A and group B （P 〉 0.05）. Conclusion：Afferent neurons of govern same side sacroiliac joint were included by L1 - S1 DRG. The same side paravetebral sympathetic trunk might be an important neural pathway between sacroiliac joint and L1,L2 DRG. However, it might be not a key bypass from sacroiliac joint to L3 - S1 DRG.