木麻黄根瘤中分离的三株弗兰克氏菌生长与培养方法的研究

Studies on Growth and Cultural Method for Three Frankia Strains from Root Nodules of Casuarina Specles

比较了从不同来源的木麻黄根瘤中分离得到的三株弗兰克氏菌（Ｆｒａｎｋｉａ）在两种浓度不同碳源培养基中的生长差异，结果表明：供试菌株在以丙酮酸钠为唯一碳源时生长最好，其生长量为其它碳源的１．３～４７．７倍；最适合的碳源浓度为０．２３ｇＣ／Ｌ在三种不同的Ｆｒａｎｋｉａ菌培养方式中，通入无菌空气及发酵培养较静置培养能显著地提高弗兰克氏菌的生长量。同时还探讨了弗兰克氏菌的发酵条件。

The effect of different carbon sources on growth of Frankia isolates for Ca-suarina sp.was studied. For each of the three isolates、JCT287,KB5 and HFPCI3,growthwas greatest on the carbon sources pyruvate;for the low carbon concentration growth wasgenerally better than that at high carbon concentration. In addition,three cultural methodsfor Frankia were compared,the results showed that culture with air supplied and fermenta-tion can increase growth of Frankia. The conditions of ferment were discussed.