纳米TiO_2——胶原复合机理探讨

Study on Mechanism of Nano-TiO_2 in Combination with Collagen

选用皮粉及山羊酸皮作为胶原替代物，采用溶胶凝胶法制备纳米TiO2——胶原复合材料，分析其结合机理。激光粒度仪测试结果表明，小粒径的纳米微粒在TiO2纳米溶胶向凝胶的转变过程中形成，溶胶中微粒的Z-平均粒径为15-70nm。复合材料中原位形成的纳米TiO2微粒，在原子力显微镜下所观察到的粒径大小约为40nm。可以看出，纳米TiO2微粒在皮胶原中原位生成后，粒径无明显变化。红外光谱分析表明，胶原可能与纳米前驱物中的钛络合物形成了某种形式的结合。DSC图谱的分析结果表明氨水的加入能促进此过程。

TiO2-collagen nanocomposite was prepared via sol-gel procedure. Hide power and pickled goatskins were used as replacers of collagen for nanocomposites. In the experiment, Malvern Zetasizer Nano ZS, Atomic Force Microscope （AFM）, Transmission Fourier Transform Infrared （FTIR） spectroscopy and Differential Scanning Calorimetry （DSC） were used to study mechanism of nano-TiO2 in combination with collagen. The results from Zetasizer indicated that nanoparticles with small size could be formed during the process from sol to gel, and the average particle sizes of nano-TiO2 in nano-precursor are 15-70 nm. Also, the ones in nanocomposite, which were produced in-situ, are about 40 nm, observed by AFM. There is no much difference between the particle sizes of nano-TiO2, whether they were prepared in the nanocomposite or not. Furthermore, FTIR spectrum showed that coordination may occur between carboxyl group of collagen and Ti complexes in nano-precursor, and DSC analyses elucidated that the addition of ammonia could strengthen the combination.