高压氧对小鼠甲状腺功能的影响

Effects of Hyperbaric Oxygen on Thyroid Function in Mice

目的:研究在高压氧条件下小鼠甲状腺功能的变化,并对其变化机理进行初步探讨。方法:实验用昆明种小白鼠,雌雄均用,实验动物随机被分为:正常对照组、0.2MPa高压氧组和0.25MPa高压氧组。用化学发光法测定小鼠血清甲状腺激素水平。结果:0.2MPa和0.25MPa高压氧作用后,甲状腺激素水平均上升,与正常对照组相比,有显著性差异(P<0.05);而0.2MPa高压氧组与0.25MPa高压氧组比较,甲状腺激素水平无显著性差异(P>0.05),各组小鼠促甲状腺素分泌水平未见明显变化(P<0.05)。结论:正常小鼠甲状腺激素在高压氧作用下分泌增加,增加的幅度与氧压变化未见关联,高压氧干预对小鼠促甲状腺素分泌水平未见明显影响。

Objective To study the possible mechanism of the influence of hyperbaric oxygenation on thyroid function in mice. Methods Fifteen mice were exposed to hyperbaric oxygen at 0.2MPa 60min daily for 10 days. Another 15 mice were exposed to hyperbaric oxygen at 0.25 MPa for the same period. The mice were sacrificed afterwards and serum T3, T4, TSH levels determined with CLIA. Results In both 0. 2MPa group and 0. 25MPa group the serum T3 and T4 levels were significantly higher than those in controls （ 15 mice） （ P 〈 0.01 ）, but levels in the two groups were not significantly different from each other （ P 〉 0.05 ）. The serum TSH concentrafions changed little after hyperbaric oxygen exposure （P 〉 0.05）. Conclusion HBO （0.2MPa or 0.25 MPa） could enhance the thyroid secretion of T3 and T4, with little effect on the level of serum TSH.