低频电磁场对大鼠主动脉平滑肌细胞骨桥蛋白基因表达的影响

Effects of low frequency electromagnetic field on the expression of osteopontin gene of the aortic vascular smooth muscle cells in rats

目的观察不同磁场强度、不同作用时间的低频电磁场(LFEMF)对培养的大鼠主动脉的血管平滑肌细胞(VSMC)骨桥蛋白(OPN)基因表达的影响,以探讨磁场是否能用于经皮冠状动脉介入治疗(PCI)后再狭窄(RS)的防治。方法用含10%小牛血清的DMEM培养液体外培养大鼠主动脉VSMC,随机分为对照组、不同磁场强度(20,40,60mT)及作用时间(10,20,30min)实验组,应用反转录-聚合酶链反应(RT-PCR)及Western Blot技术结合光密度扫描分析,观察LFEMF对VSMC的OPN表达的影响。结果各LFEMF组均明显抑制VSMC的OPN mRNA和OPN蛋白的表达,且具有磁场强度依赖性抑制作用,但无时间依赖性(P<0.05)。结论LFEMF能在基因水平上抑制VSMC的OPN的表达。

Objective To investigate the effects of low frequency electromagnetic field （LFEMF） with different intensities and periods on the expression of osteopontin （ OPN ） gene of aortic vascular smooth muscle cells （VSMC） in rats, in order to explore whether or not LFMF has protective and therapeutic effects on coronary restenosis （RS） after percutaneous coronary intervention （PCI）. Methods Rodent VSMCs were cultured with DMEM supplemented with 10% calf plasma in vitro, and divided into a control group and LFEMF groups with different exposed intensities （20 mT, 40 mT, 60 mT） and periods （10 min, 20 min, 30 min） , then the effects of LFEMF on OPN gene expression in VSMC were measured by using of RT-PCR and Western Blot analysis. Results The expression of OPN mRNA and protein was inhibited by LFEMF with different intensities and periods （P 〈 0.05 ） , and positively relative to the intensities of LFEMF, but not periods. Conclusion The expression of OPN gene was significantly inhibited by LFEMF.