脑力苏胶囊对血管性认知障碍大鼠学习记忆障碍及额叶皮层神经元作用的实验研究

Experimental study on Influence of Naolisu Capsule on Rats' Learning Abilities and Cortex Neurons of Vascular Cognitive Impairment

目的:探讨脑力苏胶囊改善血管性认知障碍(VCI)大鼠认知障碍的作用机制。方法:采用永久性结扎双侧颈总动脉法制备慢性脑灌注不足VCI动物模型。实验大鼠随机分为假手术组、模型组、喜得镇组、脑力苏胶囊大剂量组、脑力苏胶囊中剂量组、脑力苏胶囊小剂量组。用水迷宫进行学习、记忆状态测定;借助光学显微镜对大脑额叶皮层组织形态结构进行观察。结果:大、中剂量脑力苏胶囊、喜得镇治疗后VCI大鼠学习、记忆障碍得到明显改善(P<0.05);大脑皮层神经元数量显著增多(P<0.05)。结论:脑力苏胶囊具有改善VCI大鼠学习、记忆障碍的能力,其可能的作用机制为改善脑血流量,对抗缺血缺氧,阻止皮质神经元变性坏死。

Objective,To study the mechanism of Naolisu（NLS） capsule for rats＇ Vascular cognitive impairment（VCI）. Methods： Vascular cognitive impairment model was established by a 2-vessel occlusion （2-OV） method. These model rats were randomly classified into six groups, namely, model group （ MDG）. Hydergin group （ HDG）. Naolisu Capsule high-dose group （ NLS1 ）. Naolisu Capsule middle dose group （NLS2）. Naolisu Capsule low-dose group （NLS3） and sham operation group （SOG）. The learning and memory abilities of rats were estimated by water maze test. The pathomorphologic changes of cerebral cortex neurons have been observed. Results： The learning and memory abilities of rats in NLS1 and NLS2, HDG significantly promoted. The mumber and dimension were upgrade in the cerebral cortex neurons of NLS1 and NLS2. HDG. Conclusion：NLS capsule could enhance the learning and memory abilities of vascular cognitive impairment. The mechanism of NLS capsule including： regulate brain blood infusion and anti-ischemia, inhibiting neuron apotosis, stimulating neurons reproduction.