摘要
目的建立高效液相色谱法测定清肺化痰口服液中薯蓣皂苷元含量方法。方法样品进大孔吸附树脂吸附后70%乙醇洗脱,反相高效液相色谱法测定,Inertsil ODS.3(4.6nm×250nm,5μm)色谱柱,流动相乙腈-水(90:10),流速1.0ml·min^-1,进样量20μl,检测波长203nm。结果样品中薯蓣皂苷元含量测定线性范围为1,6~8.0μg,(r=0.9994),平均回收率95,32%,RSD为0.5%。结论含量测定方法简便、可靠、实用,可作为制剂的质量标准依据。
Aim To establish a method of RP-HPLC to determine diosgenin,the main effective component from rhizoma pafidis in the oral liquor. Methods The operating conditions were Inertsil ODS-3 (4.6 nm × 250 nm ,5 μm), mobile phase consisted of acetonitrile-water (90: 10) , 1.0 ml· min^-1 as flow and UV detector at 203 nm. Results The standard curve was linear in the range of 1.6×8.0μg for diosgenin. The correlation coefficient was 0.999 4. The average recovery rate and RSD were 95.32% and 0.5%, respectively. Conelusion The methods were simple, reproducible, realizable, and suitable for quality control of qingfeihuatan oral liquor.
出处
《安徽医药》
CAS
2006年第2期105-106,共2页
Anhui Medical and Pharmaceutical Journal