虎杖药材高效毛细管电泳指纹图谱研究

Study on HPCE fingerprint of Rhizoma Polygoni Cuspidati

目的:建立虎杖药材指纹图谱分析方法。方法:采用 Agilent^(3D)CE 高效毛细管电泳仪,未涂渍熔融石英毛细管(75μm×48.5cm,有效长度40.0cm),压力进样5.25Pa×3s,运行缓冲液为10mmol.L^(-1)硼酸钠溶液-甲醇(1:1),检测波长为220nm,运行电压30kV,温度25℃,内标为厚朴酚。结果:确定了虎杖药材甲醇提取部分12个共有峰,不同产地虎杖样品指纹图谱与对照指纹图谱相似度系数均在0.9以上,可用于虎杖药材的定性鉴别。结论:方法准确可靠,为提高虎杖药材质量控制标准提供参考。

Objective：To establish the fingerprint of Rhizoma Polygoni Cuspidati by HPCE. Method：Untreated fused silica capillary （48.5 cm×75 μm, effective length is 40 cm） was used. Pressure injection of 5.25 Pa × 3 s was employed, and the temperature was 25 ℃. The detection wavelength was set at 220 nm. The samples were analyzed with 10 mmol · L^-1 sodium borate -methanol（ 1：1 ） running buffer at 30 kV voltage. The internal standard substance was magnolol. Result： 12 common peaks were found in the fingerprints of Rhizoma Polygoni Cuspidati and good similarities with correlation coefficients higher than 0. 9 were found in fingerprints between the herbs from different sources and the standard fingerprint, which can be utilized for the identification of Rhizoma Polygoni Cuspidati. Condusion： The method is accurate and reproducible, providing a new reference for quality control of Rhizoma Polygoni Cuspidati.