HPLC法测定毛叶钝果寄生中3种主要黄酮成分的含量

HPLC determination of three main flavones in Taxillus nigrans(Hance)Danser

目的:建立 HPLC 法测定毛叶钝果寄生中3种主要黄酮成分的含量。方法:采用 HPLC 法,色谱柱为 Zorbax SB-C_(18)柱(4.6mm×250mm,5μm),以乙腈-甲醇-1%乙酸溶液(18:13:69)为流动相,流速为0.8mL·min^(-1),检测波长254nm,测定毛叶钝果寄生中金丝桃苷、芦丁、广寄生苷的含量。结果:金丝桃苷、芦丁及广寄生苷的线性范围分别为16.72～836,19.68～984,19.84～992μg·mL^(-1),相关系数均为0.9998。加样回收率(n=5)分别为100.1%,100.2%,101.8%;RSD 分别为3.4%,3.5%,1.7%。毛叶钝果寄生样品中3种成分的平均含量(n=5)分别为0.206%,0.069%,0.051%;RSD 分别为3.1%,3.2%,2.7%。结论:该方法准确、重现性好,可作为毛叶钝果寄生药材的质量控制标准。

Objective ：To establish an HPLC method for the determination of the contents of three main flavones in Taxillus nigrans （Hance）Danser. Methods：Contents of hyperin ,rutin ,guajeverin in Taxillus nigrans was measured by HPLC [ a column of Zorbax SB -C18 （4. 6 mm × 250 mm ,5μm） ,with mobile phase of acetonitrile -methanol - 1% acetic acid（ 18： 13： 69） ,flow rate 0. 8 mL · min^-1 ,detection wavelength of 254 nm]. Results：The calibration curves showed good linearity in the range of 16. 72 -836 μg · mL^-1 （hyperin） ,19. 68 -984 μg ·mL^-1 （rutin） and 19. 84-992 μg · mL^-1（guajeverin） ;correlation coefficients were all 0. 9998. The average recoveries（ n = 5 ）were 100. 1% ,100. 2% and 101.8% ;RSD were 3.4% ,3.5% and 1.7% ,respectively. The average contents（n =5）of the three components in Taxillus nigrans were 0. 206% ,0. 069% and 0. 051% ,and RSD were 3. 1% ,3.2% and 2. 7%. Conclusion：An accurate and reproducible method can be used for the quality control of Chinese medicine Taxillus nigrans （Hance） Danser.