温和气单胞菌单克隆抗体的制备及特性分析

Preparation and Characterization of Monoclonal Antibodies against Aeromonas Sobria

利用细胞融合技术建立了5株分泌抗温和气单胞菌CR79-1-1株单克隆抗体(McAb)的杂交瘤细胞株,特异性鉴定获得2株与参考菌株中的温和气单胞菌发生反应,并且与嗜水气单胞菌以及鳗弧菌、爱德华氏菌、克鲁氏耶尔森菌、大肠杆菌、荧光假单胞菌均不反应的杂交瘤细胞,命名为2G3、1A4;快速酶联免疫分析法测得2G3、1A4的亲和常数为5×108L/mol、1.725×108L/mol;应用方阵配对试验证实,2株单抗针对特异性抗原上不同表位。

The authors established five hybridoma cell lines against A. sobria strain CR79- 1 - 1 by fusion technique. The specificity analysis by indirect EI.ISA proved that monoclonal antibodies 2G3 and 1A4 could react with A. sobria. None of them had cross reaction with A. hydrophila, Vibrio anguillarum , Edwardsiella, Yersinia ruckeri , E. coli and P..fluorescent. Their affinity constants were 5 × 10^8 and 1.725 × 10^8 L/tool respectively, Different epitopes of A. sol）ria were recognized by 2G3 and IA4.