摘要
在pH4．10的Britton-Robinson缓冲溶液中，肝素与派罗宁B形成离子缔合物，染料发生明显的褪色，体系吸光度的降低与肝素钠浓度成正比，肝素浓度在0～50μg·（25mL）^-1范围内符合比耳定律。研究了表面活性剂和共存物质的影响，表明方法选择性好。用于肝素钠注射液效价的测定，结果令人满意。
A new spectrophotometric method for the determination of trace heparin has been studied, based on the discoloration reaction between heparin and pyronine B in the Britton-Robinson buffer solution at pH 4.10. The reduction of absorbanee is directly proportional to the concentration of heparin. The linear range of heparin is from 0～50μg·（25mL）^-1 to 50 μg·（25mL）^-1. The effects of some surfaetants and coexisting sub- stances on the reaction have been investigated and the results show that the selectivity of the method is good. The method has been applied to determine the potency of heparin sddium for injection with satisfactory results.
出处
《化学与生物工程》
CAS
2006年第2期57-59,共3页
Chemistry & Bioengineering
基金
贵阳市科技局创业基金资助项目(T2004-4)
