米非司酮对高转移卵巢癌细胞株HO-8910PM蛋白水解作用的影响

Study of effect of mifeprestone(MIF) on the expression of proteolytic enzymes of metastatic human ovarian epithelial cancer cell line HO-8910PM in vitro

目的研究高转移卵巢癌细胞株HO-8910PM基质金属蛋白酶的表达情况及米非司酮对HO-8910PM细胞水解基质蛋白作用的影响。方法应用免疫组化染色法,检测HO-8910PM卵巢癌细胞基质金属蛋白酶的表达情况;采用水解空斑法检测不同浓度米非司酮对HO-8910PM细胞的体外水解人血浆基质蛋白空斑的变化。结果HO-8910PM细胞MMP-10及MMP-9表达较高,MMP-2表达较低;10μmol/L、20μmol/L米非司酮培养48小时可显著降低具有蛋白水解作用的HO-8910PM细胞的百分比(P<0.01),而5μmol/L米非司酮组无明显变化(P>0.05);不同浓度的米非司酮(5μmol/L、10μmol/L、20μmol/L)明显缩小HO-8910PM细胞的平均水解空斑面积。结论HO-8910PM细胞可表达MMP-10、MMP-9、MMP-2;米非司酮可抑制HO-8910PM细胞体外水解基质蛋白的能力。

Objective To study the expression of MMPs and the effect of hydrolization of mifepristone on matrix protein （MP） of the high metastatic human ovarian epithelial cancer cell line HO- 8910PM in vitro. Methods Immunohistochemical technique was used to measure the MMP- 2,MMP- 9,MMP- 10 expression of HO - 8910PM cells. The proteolytic spots of HO - 8910PM cells were examined with mifeprestone of different concentration. Results The expression of MMP- 10 and MMP - 9 were obvious and the MMP - 2 were less in the kytoplaam of HO - 8910PM. The number of proteolytic spots were decreased obviously with mifeprestone （ 10 μmol/L,20 μmol/L） for 48 h （ P 〈 0.01 ）, but the concentration of 5μmol/L had not significant changes （P 〉 0.05）. The average areas of preteolytic spots was shrinked significandy with mffeprestone （5μmol/L, 10 μmol/L,20 μmol/L） （P〈0.01）. Conclusion HO- 8910PM can express MMP- 10,MMP- 9 and MMP- 2; mifeprestone can inhibit the proteolysis of HO - 8910PM cells in vitro.